Completed Research works

Though the Institute is at its infant stage, members of the Institute are conducting few valuable research activities. Currently, the Institute staffs in group have submitted research proposals for the University Research and Publication Office, the Federal Ministry of innovation and technology, Ethiopian Biotechnology institute. The following works were completed by the grant obtained from University of Gondar;-

Completed Research works
  1. Assessing the availability and impact of GM crops on health and environment around the Ethio-Sudan trans-boundary region
  2. Polymorphisms and frequency of rpoB, Kat and inha genes in MDr TB HIV- co infected patients in North West Amhara region (supported by University of Gondar.
  3. Frequency and Effect Of CCR5 Alleles on HIV-1 Disease Progress among North and South Gondar HIV-1 untreated Seropositive Individuals
  4. Detection, Isolation and control of Ginger pathogenic organism production of clean disease free planting material from selected cultivars, genetic diversity analysis and improvement of post harvest system in Ginger (Zingiver officinale) (Supported by University of Gondar)
  5. The role of PPAy-2 gene polymorphism on risk of Diabetic 2 patients from Gondar University Teaching Hospital.
  6. The role of TNF-308 gene variant and susceptibility to Plasmodium  falciparum in Denbia  wereda malaria infected   children, North Gondar, Ethiopia .
  7. Molecular characterization of Integerons from clinical isolates of Staphyloccus species

obtained from patients visiting Gondar University teaching hospital.

  1. Antibacterial activity of honey of stingless bee (Apis mellipodae) tenegn (Trigona

iridipennis and honeybee (Apis mellifera alone and in combination with garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria.

  1. Isolation of Mycobacterium species from slaughtered cattle lesion in Gondar abattoir and drug susceptibility.
Ongoing research works
  1. Improving the quality of Sesame through Molecular Breeding to ensure food security
  2. Isolation and characterization insecticidal proteins from Bacillus thuringiensis from soil for controlling major malaria vectors from malaria endemic parts of Amhara Region.
  3. Isolation and exploring the phylogeny and plant growth promoting capacity of nitrogen fixing and phosphate solubilizing bacteria in Amhara and Afar region
  4. Evaluation of Malaria transmission status in relation to ITN use practice, vector breeding potential pattern and allele frequency of Insecticide resistance among major malaria vector anopheles in Malaria endemic region of North West Ethiopia (supported by University of Gondar)
  5. Production of honey wine (Tij) at pilot and commercial scale using isolated Saccharomyces cervisiae in aseptic condition (supported by Ethiopian Biotechnology Institute)
  6. Optimization and scale up process for production of petroleum from available up process for production petroleum from available clean biological substances through the application of biotechnological technique (supported by The Federal ministry of Science and technology)
  7. Isolation and characterization of bacterial community and assessment of their potentials in removing nitrogenous wets (NH4 , NO3 and NO2) from municipality and industrial west water system around Gondar and Bahirdar (Supported by University of Gondar)
  8.  
Course materials, Lab manuals being prepared:

Lists of teaching materials approved by the Research and Publication Office (RPO).

Sr. No.

Title of the Teaching Material

Cr. Hrs

Course Code

Responsible Person

Status

1

Enzyme Technology             

3

Biot. 344

Mr. Abrham Abera

 

2

Animal Biotechnology

3

Biot.431

Mr. Atekelt Alemayehu

 

3

Policy and Safety Issues in Biotechnology

2

Biot. 453

Mr. Getnet Dino

 

4

Cell and Molecular Biology. (Textbook for Under Graduate students).

 

–

Dr. Birhanu Andualem

Approved

5

Cell Biology Laboratory manual

 

Biol 203
Representative publications

Members of the Institute have more than 80  publications as of January 2018.

 

  1. M Birhan, M Temesgen, A Shite, N Berhane, M Bitew, E Gelaye, T Abayneh, B Getachew

Seroprevalence and Associated Risk Factors of Infectious Bronchitis Virus in Chicken in Northwest Ethiopia

 

  1. Mastewal Birhan, Nega Berhane, Molalegne Bitew, Esayas Gelaye, Belayneh Getachew, Aragaw Zemene, Kassahun Birie, Wudu Temesgen, Takele Abayneh./ Sero-epidemiology of Marek’s disease virus on local and exotic chickens in the northwest Ethiopia

 

  1. Yakob Gebregziabher Tsegay, Molalegne Bitew, Tigist Workneh, Assegdew Atlaw, Mintsnot Aragaw, Mesay Gemechu, Nega Brhane. The level of liver and renal function biomarker abnormalities among hospitalized COVID-19 patients in Ethiopia.

 

  1. Endeshaw Chekol Abebe, Tekeba Sisay, Nega Berhane, Snr Tesfahun Bekele, Tadesse Asmamaw Dejenie. Current Applications and Future Perspectives of CRISPR-Cas9 for the Treatment of Lung Cancer

 

  1. A Seid, N Berhane. Molecular mechanisms of genetic interaction (epistasis) in the evolution and management of antibiotic resistance tuberculosis current consequence and future Perspectives. International Journal of Pathogen Research 2021

 

  1. Aynias Seid, Nega Berhane, Takele Abayneh, Solomon Tesfaye. Microbes and Infectious Disease 2020.

 

 

  1. Mastewal Birhan, Ashenafi Syoum, Saddam Mohammed Ibrahim, Tewodros Fentahun, Addisu Mohammed, Nega Berhane, Molalegne Bitew, Esayas Gelaye, Malede Birhan Atanaw, Belayneh Getachew, Bereket Dessalegn, Anmaw Shite Abat, Kassahun Berrie,

 

  1. Kassaye Adamu, Takele Abayneh. Serological Evidence of Infectious Laryngotracheitis Infection and Associated Risk Factors in Chickens in Northwestern Ethiopia. The Scientific World Journal 2022

 

  1. Aynias Seid, Nega Berhane, Semira Nureddin. Frequency of rpoB, katG, and inhA Gene Polymorphisms Associated with Multidrug-Resistant Mycobacterium tuberculosis Complex Isolates among Ethiopian TB Patients: A Systematic Review Interdisciplinary Perspectives on Infectious Diseases 2022.

 

 

  1. Sefinew Tilahun, Marye Alemu, Mesfin Tsegaw, Nega Berhane. Morphological and Molecular Diversity of Ginger (Zingiber officinale Roscoe) Pathogenic Fungi in Chilga District, North Gondar, Ethiopia

 

  1. Hawi Mohammed, Fatuma Mohammed, Preetha Velaydhanpillai, Nega Berhane, Aragaw Zemene. Synergistic Antibacterial Activity of Black Seed (Nigella sativa) and Clove (Syzigium Aromaticum) Against Some Selected Pathogenic Bacteria. Materials Research 2022

 

 

  1. Mastewal Birhan, Nega Berhane, Molalegne Bitew, Esayas Gelaye, Belayneh Getachew, Takele Abayneh. Structural Equation Modeling and Management Role On The Control of Marek’s Disease Outbreaks in Amhara Region Ethiopia. 2021

 

  1. Dessie Eshetie, Minichil Worku, Abiye Tigabu, Melak Aynalem, Nega Berhane. Gen X-Pert MTB/Rif Assay with the Ziehl Nielsen, and Florescence Microscopy Detection Rate of Urinary Tuberculosis Among Suspected and Pulmonary MTB Positive Patients Visiting University of Gondar comprehensives specialized teaching hospital. 2021

 

 

  1. Abdifitah Abdullahi Abdi, Nega Berhane, Aragaw Zemene. Evaluation of In-Vitro Anti-Bacterial Qualities of Camels Urine in Somali Region of Ethiopia against Selected Bacterial Pathogens. The International Journal of Biotechnology 2021.

 

  1. Yakob Gebregziabher Tsegay, Molalegne Bitew, Assegdew Atlaw, Mintsnot Aragaw, Shambel Araya, Mesay Gemechu, Nega Brhane. Lipid Profile Abnormalities and Their Association with Covid-19 Severity among Patients Admitted at Covid-19 Center in Ethiopia. Journal of Clinical & Biomedical Research 2021.

 

 

  1. Meseret Ayelign, Melak Aynalem, Nega Berhane. Hepatitis and HIV Co-infection at University of Gondar Specialized Referral Hospital: Northwest Ethiopia. Hepatic Medicine: Evidence and Research 2021.

 

  1. Meera Indracanti, Nega Berhane, Tigist Minyamer. Factors Associated with Pre-and Post-Educational Intervention Knowledge Levels of HPV and Cervical Cancer Among the Male and Female University Students, Northwest Ethiopia. Cancer Management and Research 2021.

 

 

 

 

  1. Kibrnesh Tegenaw Tsega, Kagira Maina, Nega Berhane. Characterization of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Ethiopian Rhode Island Red Chicken and their Potential Use as Probiotics
  2. Firnus Haile, Mequanente Dagnaw, Nega Berhane. A review on regulatory roles of micro RNA and micro RNA based therapeutics for diabetics mellitus. 2021

 

 

  1. Kibrenesh Tegenaw Kohn Kagira, Nega Berhane. Probiotics and Poultry Gut Microflora. 2021.

 

  1. Emebet Kibkab, Nega Berhane and Tekeba sisay. isolation, characterization and

evaluation of symbiosis efficacy of nitrogen fixing bacteria on dosha faba bean from

soil collected in selected districts of amhara and afar regions, ETHIOPIA. World

journal pharmacy and pharmaceutical Science. 2020;10(2):1693-1727.

22 Ayelegn B, Genetu M, Wondemaggne T, Adan G, Negash M, Berhane N. TNF-α

(−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes

Patients. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

2019;12:2453-2459.

  1. Tekeba Sisay, Nega Berhane and Deepak Kumar. Derug resistance mechanism and

molecular Diagnostics method of Tuberculosis. Molecular Biology open

access.2019;8(2):1-8.

  1. Nega Berhane, Tadele Tamiru, Wagaw Swndku and Desalegh Mengesha. Association

of Angiotensin Converting Enzyme (ACE) Gene Polymorphisms and Risk of Diabetic 2

Among Patients Visiting Bahirdar Felegehiwot Referral Hospital North West, Ethiopia.

Molecul Biology 2018;7(02):

  1. Mahlet shiferam Berhanu Andualem and Nega Berhane. Isolation and

characterization of Saccaromyces cervisiae yeasts isolates from “tella” for beer

production. annual research and review in biology 2017;

  1. Nega Berhane and Mahlet Tegegne. Association of tatA and tatB genes and

multidrug resistance pattern of S.ourius among patients visiting Gondar University

hospital. Biotechnology International 2016; 9(10):194-208.

  1. Deepak Kumar Verma, Nega Berhane, Wagaw Senedeku.Cell mediated immune

response against fractionated Lipopolysaccharide (LPS) antigens of Brucella

Meilitensis 16M in cell culture. Biotechnology international. 2016;9(9):185-193.

 

  1. Adugnaw Admasu and Nega Berhane. The ferequency and Effect of CCR5 delta 32

Allelle in Gondar population. Molecular Biology 2016;5:168

 

  1. Kibernesh Tegenaw, Nega Berhane, Wagaw Sendeku, Mulugeta aemero and

Desalgne Mengesh Tumer necrosis factor alpha-308 gene polymorphism and risk of

Plasmodium falcifarum malaria among people living in Dembia woreda, North west

Ethiopia. Biotechnology International 2016: 9(5) 122-132

  1. Aragaw Zemen and Nega Berhane. The antimicrobial effect of Lipidium sativum

exteract on some clinical isolated bacteria from university of Gondar teaching

hospital. Biotechnology international 2016;9(3)69-82.

  1. Depak Kumar Verma, Nega Berhane and Wagaw Sendeku. Nitric oxide synthesis

from activated monocytes induced by Eractionated soluble (cytoplasmic) antigen of

  1. melitensis 16M in cell culture. Biotechnology International 2016;9(4) 83-87.
  2. Nega Birhane and Tigist Meniyamer. Prevalence of Class I and class II Integerons in

multi drug resistance bacteria isolated from keha and shinta rivers in Gondar town,

North west Ethiopia. Biotechnology International 2015;8(4):118-131

  1. Wagaw Sendeku, Bewket Alefew, Dejenie Menigestu, Kassahun Seifu, Shito Girma,

Elsabet wonimu, Garoma Bekuma, Deepak Verma and Nega Berhane. Antibiotic

activity of Croton Macrostachyus agains selected pathogenic Bacteria. Biotechnology

International 2015;8(1):11-20.

  1. Tekeba Sisay and Nega Berhane. RpoB gene polymorphism and its association with

Multi drug resistance pattern of Mycobacterium tuberculosis and associated risk

factors among TB patients. J.Genet Disor Geneti Rep 2015;4:2

  1. Yalemworek Ewenetu, Wossenseged Lemma, Nega Berhane. Synergetic

antimicrobial effects of Mixture of Ethiopian Honey and Ginger powder exteracts on

standared and resistance clinical Bacterial isolates. Evidence Based complementary

alternative medicine 2014.

  1. Yalemworek Ewenetu, Wossenseged Lemma, Nega Berhane Antibacterial effect of

APis Mellifera and stingless bees honey on susptable and reistance strains of

Escherichia coli, Staphyloccocus aureus and Klebsiella pneumonia in Gondar, North

west Ethiopia. BMC complementary and Alternative medicine 2013;13:269.

  1. Nega Berhane and Wagaw Sendeku.Existance of intgeron I and II genes from clinical

isolate Staphyloccocus aureus and their role in drug resistance. Research and review

journal of Micro biology and virology. 2014;4(3):18-25

 

  1. Assessment of the effectiveness of PMTCT on children born from HIV-1 positive

mothers through Molecular Diagnosis. Research and review journal of Micro biology

and virology2016;6(1):1-8

  1. Nega Berhane. Impact of PPAR-γ2 pro12Ala Gene Polymorphism and

Susceptibility of Type 2 Diabetes Mellitus in Patients Visiting Gondar University

Teaching Hospital. Research & Reviews: A Journal of Microbiology and Virology 2015;5(2):22-28

  1. Berhanu Andualem and Nega Berhane. Combined antimicrobial effect of Honey Bee

(Apies Meliifera ) and Garlic (Allium Sepa) extract against standard and clinical

pathogenic bacteria. Research & Reviews: Journal of Microbiology and Virology 2013;1:18

  1. Nega Berhane, Rabinder chandera Sobti, Shiferaw Melesse, Salih Abdul Mahdi, A.

fework Kassu. Significance of Tumor necrosis factor alpha (G/A) gene polymorphism

in the development of prostate cancer. Molecular Biology Reports 2012:

39(12):11125- 30

  1. RC sobti, VL Sharma, AM Abitew, Nega Berhan, SA MAhid, Maskari, VS Kuttat. e-

137G/C polymorphism of interlukin 18 promoter and risk of HIV-1 infection nad its

progeression to AIDS. Acta virologica 2011.

  1. RC Sobti, Sharma VL, Abitew AM, Nega Berhane, MAhdi SA, Askari M, Kuttat

VS, Wanchu A. Il-18 gene promoter region 607 C/A polymorphism in

HIV infected north Indian Population. BJMG 2011:14(2): 41-48.

  1. Nega Berhane, Rabinder Chandera Sobti and Salih Abedul Mahdi. DNA repaire

genes polymorphism (XPG and XRCC1) and association of prostate cancer in a north

Indian population. Molecular Biology Reports 2012;39(3):3251-3257.

  1. Rabinder Chandera Sobti, Nega Berhane, Salhi Abdul Mahdi, Rupinder Kler Seyd

Ali Hussieni, Vijish Kutat, Ajay Wanch. Impact of ERCC2 gene polymorphism on

HIV_1 disease progeression to AIDS among north Indian HIV patients. Mol Biology

reports 2011; 38(5):2945-52

  1. Rabinnder Chandera Sobti, Nega Berhane, Salih Abdule Mahdi, Hitnder Thakur,

Ajay Wanchu. Association of Interlukin 4 VNTR polymorphism and HIV/AIDS in a

north Indian Population. Molecular Biology Reports 2012;39(3):3251-7

  1. Sobti RC, Berhane Nega, Mahedi SA, Kler R, Hosseini SA, Kuttiat V, Wanchu A..

Association and impact of XPG Asp1104 HIS gene polymorphism in HIV-1 disease

progression to AIDS among north Indian HIV seropositive individuals. Molecular

Biology Reports 2010; 37:317-324

  1. Sobti RC, Berhane Nega, Mahedi SA, Kler R, Hosseini SA, Kuttiat V, Wanchu A.

Polymorphisms of IL-6 174 G/C, IL-10 -592 C/A and risk of HIV/AIDS among

North Indian population. Molecular and Cellular Biochemistry 2010;337:145-152

  1. Rabinder Chandera Sobti, Salhi Abdul Mahdi, Nega Berhane, Rupinder Kler Seyd Ali

Hussieni, Vijish Kutat, Ajay Wanch. The Influence of Variations in the DNA Repair

(XRCC1) Gene on HIV-1/AIDS among Indian Population. Folia Biologica (praha)

2009:55:183-186.

  1. Rabinder Chandera Sobt,i Salhi Abdul Mahdi, Nega Berhane, Rupinder Kler Seyd Ali

Hussieni, Vijish Kutat, Ajay Wanch. Insights into the Role of IL-12B and IFN-γ

Cytokine Gene Polymorphisms in HIV-1/AIDS Infection. Folia Biologica (Praha)

2010;56: 110-115

Future plans

One of the University of Gondar transformation agenda is transforming undergraduate programs into postgraduate programs. In line with this the four existing departments are striving in expanding postgraduate study. Among the planned expansions;- M.Sc. in Molecular Biology and Bio informatics, M.Sc. in Plant Biotechnology, M.Sc. in Animal Biotechnology, M.Sc. in forensic Biotechnology programs  will get realized in a very short period of time.

The existence of an institute like Biotechnology needs to have an impact on the surrounding population through conducting problem solving researches. At this juncture, apart from the researches done so far the institute has short and long term plan in conducting research on the basis of societal priorities. Amongst them:-

  1. Developing drought, disease and insect resistance crops for increasing productivity and ensuring food security
  2. Conserving the endangered fauna and flora of the Semien Mountaind national park through Biotechnological methods
  3. Studying the flora and biodiversity composition of Alatish park
  4. Looking for renewable alternative energy resource
  5. Developing rapid diagnostic methods for infectious diseases like malaria and Tuberculosis
  6. Designing vaccines for non-curable diseases

The institute intended to convert accomplished successful research out comes into community services

In due time those exemplary and successful community service projects will be transformed into technology transfer ones besides others that will be designed by staff based upon existing problems of the society.